Age and Growth of Chub Mackerel （Scomberjaponicus） in the East China and Yellow Seas Using Sectioned Otolith Samples     

LI Gang  CHEN Xinjun  FENG Bo 《中国海洋大学学报(英文版)》2008,7(4)

Although chub mackerel (Scomberjaponicus) is a primary pelagic fish species,we have only limited knowledge on itskey life history processes.The present work studied the age and growth of chub mackerel in the East China and Yellow Seas.Agewas determined by interpreting and counting growth rings on the sagitta otoliths of 252 adult fish caught by the Chinese commercialpurse seine fleet during the period from November 2006 to January 2007 and 150 juveniles from bottom trawl surveys on the spawn-ing ground in May 2006.The difference between the assumed birth date of 1 st April and date of capture was used to adjust the agedetermined from counting the number of complete translucent rings.The parameters of three commonly used growth models,the vonBertalanffy,Logistic and Gompertz models,were estimated using the maximum likelihood method.Based on the Akaike InformationCriterion (AIC),the von Bertalanffy growth model was found to be the most appropriate model.The size-at-age and size-at-maturityvalues were also found to decrease greatly compared with the results achieved in the 1950s,which was caused by heavy exploitationover the last few decades.  相似文献   

鳗弧菌W-1对花鲈鱼苗致病性的初步研究   总被引：1，自引：0，他引：1       下载免费PDF全文

肖慧  唐学玺  陈吉祥  张培玉  蔡恒江 《海洋科学》2008,32(3):36-39

为研制和生产鲈鱼苗疫苗提供依据,采用人工感染试验方法测试了花鲈(Lateolabrax japonicus)烂尾、出血病病原菌W-1菌株(鳗弧菌Vibrio anguillarum)对花鲈苗的半致死浓度(LD50)及不同保存条件下的W-1菌株的致病力。试验结果表明:W-1菌浴感染花鲈苗的半致死浓度为2.5×107.5个/mL,针刺后菌浴感染花鲈苗的半致死浓度为2.5×107个/mL;该病原菌为一条件致病菌,创伤感染是主要的致病途径;常温多次传代、15℃、-20℃和-80℃等不同保存条件下W-1菌株的致病力均很强,但常温多次传代保存的菌株致病力比其他3种方法保存的菌株致病力稍差一些。  相似文献   

基于线粒体控制区序列的黄海日本鳀(Engraulis japonicus)的群体遗传结构*     

邹聪聪  王丽娟  吴志昊  尤锋 《热带海洋学报》2021,40(5):25-35

遗传多样性和群体遗传结构是研究群体动态变化的重要内容, 也是种质资源评估与保护的基础。日本鳀(Engraulis japonicus)是我国东部近海的重要鱼种, 具有重要的生态价值和经济价值。文章利用线粒体控制区全序列分析了黄海海域4个地理群体(北黄海北部、北黄海南部、南黄海北部和南黄海中部)日本鳀的遗传多样性、种群遗传结构和历史动态变化。结果显示, 131个样品检测到了126个单倍型, 且4个群体的单倍型多样性均很高, 其中北黄海南部群体的最低(0.995±0.009), 南黄海中部群体的最高(1.000±0.014)。而核苷酸多样性均较低, 为0.010±0.005 ~ 0.011±0.006。主成分判别分析(DAPC)和遗传分化系数F_st表明4个群体无明显的群体分化现象, 群体间的遗传同质性水平高, 分子方差分析(AMOVA)也显示分子变异基本来自于群体内, 并且没有明显的群体遗传结构。贝叶斯系统发育树分析发现, 黄海日本鳀有2个谱系, 谱系1和谱系2的分化时间为0.701Ma前, 可以追溯到更新世期间; 进一步中性检验和核苷酸错配分布分析表明这2个谱系可能发生过群体扩张。贝叶斯天际线图则显示黄海鳀鱼的有效群体数量在0.150Ma前发生了明显的下降。  相似文献   

Genetic variation in two sea cucumber （Apostichopus japonicus） stocks revealed by ISSR markers     

姚冰  胡晓丽  包振民  陆维  胡景杰 《中国海洋湖沼学报》2007,25(1):91-96

Sea cucumber Apostichopus japonicus samples were collected in Changdao, Penglai (PL), 27 individuals, and Lingshandao, Qingdao (QD), 30 individuals, in the Shandong Peninsula, China. Ten SSR primers were used to assess the genetic variation and relationship between and within the two stocks. Respectively, for each stock, the percentage of polymorphic bands was 85.2% and 86.9%; the gene diver- sity was 0.360 5 and 0.342 8; and the Shannon’s information index was 0.515 0 and 0.499 0. At species level, the percentage of polymorphic bands was 92.2%, the total gene diversity was 0.378 9 and the Shannon’s information index was 0.550 4. The coefficient of overall genetic differentiation and the ge- netic distances between the stocks were also calculated to be 0.073 0 and 0.079 6 using the POPGENE program. Results show that the genetic diversity of the two stocks is still large but the genetic distance between the two stocks is close. A dendrogram was constructed for the 57 individuals from the two stocks, showing that the genetic structure was unitary for PL stock but complex for QD stock.  相似文献   

鲐和蓝点马鲛骨骼系统的比较     

李仲辉 《广东海洋大学学报》2000,20(3):1-7

比较了鲐 Pneumatophorus japonicus ( Houttuyn)和蓝点马鲛 Scomberomorus niphonius( C.etV.)的骨骼特征。鲐脑颅整体特别是前 1/ 2部分狭长 ,蓝点马鲛脑颅较扁宽。鲐脑颅的中筛骨、鼻骨、额骨、犁骨、围眶骨等 ,咽颅的前上颌骨、上颌骨、腭骨、齿骨、角舌骨、尾舌骨、下咽骨等 ,肩带的上后颞骨、后匙骨和腰带骨等与蓝点马鲛相应部分的骨骼皆有重要差异 ,有些特征可能是科或属的区别  相似文献   

Seasonal biochemical changes in composition of body wall tissues of sea cucumber Apostichopus japonicus     

�߷�  ��ǿ  ����� 《中国海洋湖沼学报》2011,29(2):252-260

Seasonal variation in proximate,amino acid and fatty acid composition of the body wall of sea cucumber Apostichopus japonicus was evaluated.The proximate composition,except for ash content,changed significantly among seasons(P,0.05).Alanine,glycine,glutamic acid and asparagic acid were the most abundant amino acids.Total amino acid and essential amino acid contents both varied clearly with seasons(P,0.05).16:0 and 16:1n7 were the primary saturated fatty acid(SFA) and monounsaturated fatty acid(MUFA) respect...  相似文献   

Effects of photoperiod on daily activity rhythm of juvenile sea cucumber, Apostichopus japonicus (Selenka)     

董贯仓  董双林  田相利  王芳 《中国海洋湖沼学报》2011,29(5):1015-1022

In nature, Apostichopus japonicus exhibits a behavioral characteristic of emerging at night and sheltering during the day. Hence, it was commonly believed that longer and darker light conditions are better for a population of A. japonicus. In aquaculture situations therefore, animals have commonly been cultured without lighting, especially during juvenile-hatching culture. However, how the length of darkness each day affects A. japonicus and what the mechanism is of behavioral response of A. japonicus to different photoperiods remain unclear, and are specifically addressed in this study. We applied nine photoperiod treatments (light:dark (LD) cycles at 0:24, 3:21, 6:18, 9:15, 12:12, 15:9, 18:6, 21:3, and 24:0 under 500 lx) to 60 individuals (29.73 ± 0.23 g) per treatment (with 3 replicates). The environmental conditions were set as: water temperature = 16.9 ± 0.3°C, dissolved oxygen>6.0 mg/L, ammonia<0.3 mg/L, pH = 7.8-8.2, and salinity = 30-32. Under experimental conditions of continuous darkness as well as continuous light, cyclic nocturnal activity patterns of A. japonicus (viz. the animals emerged and fed at night and sheltered during the day) were observed. However, they spent more and less time, respectively, moving and feeding under continuous darkness and continuous light, than those under a natural light cycle. Under photoperiods with 6-12 h of light, the animals showed one sheltering behavior transition and two emerging behavior transitions. These behavior transitions appeared to be governed by some internal physiological factors and induced by daily light variation. The behavior of the animals was significantly affected by different photoperiods, and the distribution rate (DR) of the animals emerging at "daytime" increased with lengthening "light time" (except for the LD 3:21 cycle). We also found that there was no significant difference in mean DR per day under LD cycles of 6:18, 9:15, 15:9, and 12:12. Mean DRs per day were significantly lower for these photoperiods, than for those under LD cycles of 0:24 and 3:21, and significantly higher than for those under LD cycles of 18:6, 21:3 and 24:0. These results imply that 6-15 h light per day were the similar photoperiods to natural sunlight. From the viewpoint of activity and feeding behaviors, these might be the optimal photoperiods for rearing A. japonicus.  相似文献   

南流江中上游毛蟹调查初报     

谭红  陈德桢  黄本佳  陈家靖  刘小军 《广东海洋大学学报》1991,(2)

对北部湾南流江水系的日本绒螯蟹合浦亚种(Eriocheir japonicus hepu ensis Dai 1900)的形态特征、生态习性、分布状况、捕捞运输、经济价值、资源利用作了阐述,并对如何加强资源保护提出4点建议。  相似文献   

花鲈类胰岛素生长因子-1基因的全长cDNA分离与表达分析   总被引：1，自引：0，他引：1  

钱焜  温海深  迟美丽  倪蒙  张冬茜  丁玉霞 《中国海洋大学学报(自然科学版)》2014,44(2)

采用逆转录聚合酶链式反应(RT-PCR)和cDNA末端快速扩增法(RACE)获得花鲈(Lateolabrax japonicus)类胰岛素生长因子-1(IGF-1)的cDNA全长序列,利用荧光实时定量PCR技术研究了花鲈IGF-1mRNA在成鱼各组织中的表达特征。结果显示,花鲈IGF-1cDNA全长序列为873bp,编码区序列为555bp,编码186个氨基酸,推测成熟氨基酸分为6个结构域,分别是信号肽区域,A、B、C、A、D区域和E肽。花鲈IGF-1氨基酸序列与其他海水鱼类的IGF-1相似度较高,与淡水鱼类相似度较低,与高等脊椎动物相似度更低,说明海水鱼类的IGF-1在进化过程中发生了较大的变化。组织表达分析显示,花鲈IGF-1mRNA在肝脏中表达量最高,在肠、盲肠、肌肉、脾脏中次之;在鳃、垂体、性腺、胃、肾脏、脑、头肾、心脏中表达量较低。本文为鱼类IGF基因功能研究奠定基础,为花鲈生长调控提供科学依据。  相似文献   

海产花鲈IGFBP-1、2基因的克隆及表达分析     

钱焜  温海深  迟美丽  倪蒙  张冬茜  丁玉霞 《中国海洋大学学报(自然科学版)》2014,44(9):37-45

利用逆转录聚合酶链式反应(RT-PCR)和cDNA末端快速扩增法(RACE)克隆得到花鲈的IGFBP-1和IGFBP-2基因cDNA全长序列,IGFBP-1全长1 779bp,编码248个氨基酸,IGFBP-2全长815bp,编码267个氨基酸。花鲈的IGFBP-1和IGFBP-2氨基酸序列同其他硬骨鱼类一样都包括C-端结构域、N-端结构域和多变的中央L-结构域。二者均存在18个保守的半胱氨酸残基以维持氨基酸二级结构的稳定,同时也都拥有N-端结构域的CGCCXXC-box和C-端结构域的CWCV-box,它们都是与IGF配体结合的重要结构。此外,IGFBP-2的C-端结构域还存在一个RGD结构,它能够与整合素相互作用,介导细胞与细胞之间、细胞与细胞外基质之间的黏附作用。利用荧光实时定量PCR技术对花鲈的脑、垂体、心脏、鳃、胃、盲肠、肠、头肾、肾脏、肝脏、性腺(雄)、脾脏和肌肉这13个组织的IGFBP-1和IGFBP-2mRNA的相对表达水平进行分析。结果显示,花鲈IGFBP-1mRNA表达量较为广泛,在肝脏中的转录水平最高,在肌肉和脾脏有较多的表达。花鲈IGFBP-2mRNA仅在肝脏中的表达水平最高,在肌肉中表达水平较高,其他组织表达量较低。  相似文献